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Characterization and purification of the neuronal sodium-ion-coupled 5-hydroxytryptamine transporter
D Graham1, H Esnaud, S Z Langer
1Department of Biology, Synthélabo Recherche (L.E.R.S.), Paris, France.
Biochemical Society Transactions
|February 1, 1991
Abstract:
The selective 5-hydroxytryptamine (5-HT) uptake inhibitor, [3H]paroxetine, has been used as a specific probe in a series of experiments aimed at characterizing the substrate and 5-HT uptake inhibitor binding domains on the neuronal sodium-ion-coupled 5-HT transporter. In addition, an affinity-chromatographic protocol which provided a greater than 3000-fold purification of this neuronal transporter protein is outlined.