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Updated: Jul 7, 2026

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
09:30

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Published on: December 30, 2014

Identification of proteomic differences in asthenozoospermic sperm samples.

Juan Martínez-Heredia1, Sara de Mateo, José M Vidal-Taboada

  • 1Human Genetics Research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Casanova 143, 08036 Barcelona, Spain.

Human Reproduction (Oxford, England)
|February 19, 2008
PubMed
Summary

Researchers identified 17 differentially expressed proteins in asthenozoospermic (reduced sperm motility) semen samples. These protein markers may aid in diagnosing male infertility and understanding its causes.

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Area of Science:

  • Reproductive Biology
  • Proteomics
  • Male Infertility Research

Background:

  • Asthenozoospermia is a common cause of male infertility with largely unknown etiology.
  • Proteomic analysis offers a method to identify proteins associated with asthenozoospermia.

Purpose of the Study:

  • To compare sperm protein expression in asthenozoospermic individuals versus controls.
  • To identify potential protein biomarkers for asthenozoospermia.

Main Methods:

  • Two-dimensional proteomic analysis was used.
  • 101 sperm protein spots were analyzed in 20 asthenozoospermic samples and 10 donor controls.

Main Results:

  • Seventeen protein spots showed differential expression in asthenozoospermic samples.

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Last Updated: Jul 7, 2026

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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Published on: December 30, 2014

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Published on: October 9, 2013

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  • Identified proteins include cytoskeletal actin-B, annexin-A5, and heat shock protein-HSPA2.
  • Protein expression levels allowed for unsupervised clustering of asthenozoospermic samples.

    • Conclusions:

    • Several proteins were found at altered levels in asthenozoospermic sperm.
    • These proteins are potential candidates for diagnostic markers of male infertility.
    • Further research can elucidate pathogenic mechanisms of asthenozoospermia.