The reliability of fat

The exercise intensity eliciting maximal fat oxidation (Fat(max)) is typically determined during an incremental test. Its reproducibility, however, has not been thoroughly investigated so far. To address this issue, 21 healthy subjects (23.5+/-1.7 years; BMI 22.4+/-1.8 kg/m(2); VO(2peak) 47.4+/-11.3 mL/min/kg) carried out two identical cycling tests to determine Fat(max) after an initial incremental baseline test. The duration of each of five stages during the Fat(max) tests was 6 min. The first stage equalled the first increase in blood lactate during the baseline test; the highest stage corresponded to a respiratory exchange ratio of 1.00. Between these intensities the other three stages were distributed evenly. Fat(max) was 28.0+/-8.6 L/min (59.2+/-18.1% VO(2peak)) in the first test and 29.8+/-10.5 L/min (62.9+/-22.2% VO(2peak)) in the second one. There was no significant difference between both Fat(max) determinations [number of stage: P=0.31; total VO(2): P=0.20; VO(2) utilized for fat oxidation (VO(2Fat)): P=0.33]. Linear correlation coefficients between tests were r=0.84 (total VO(2); P<0.001) and r=0.83 (VO(2Fat); P<0.001). However, Bland-Altman plots revealed wide 95% limits of agreement of 0.91 L/min (total VO(2)) and 0.32 L/min (VO(2Fat)). In conclusion, spontaneous intraindividual variability in Fat(max) appears too large to recommend the use of this parameter for the prescription of training intensity.