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Detection of papillary thyroid carcinoma with serum protein profile analysis
William H Moretz1, Christine G Gourin, David J Terris
1Department of Otolaryngology-Head and Neck Surgery, Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta, GA 30912-4060, USA.
Objective:
To determine the sensitivity and specificity of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) for papillary thyroid carcinoma (PTC) detection.
Design:
The SELDI-TOF-MS protein profiles of patients with PTC, patients with benign nodular disease (BND), and healthy controls were analyzed to determine the sensitivity and specificity of SELDI-TOF-MS assay for PTC detection. Data analysis was performed to process the spectral data and classify the disease status of the patients.
Setting:
Academic tertiary care hospital.
Patients:
Serum samples were collected prospectively from 7 patients with PTC, 8 patients with BND, and 7 healthy control volunteers.
Intervention:
All patients diagnosed as having PTC or BND underwent thyroidectomy from October 21, 2004, to January 31, 2006.
Main Outcome Measures:
Twenty-two serum samples were analyzed.
Results:
Most protein peaks resolved by the SELDI-TOF-MS assay were in the range of 1 to 20 kDa. Classification tree analysis based on peak expression distinguished patients with PTC from those with BND with 85.7% sensitivity and 100% specificity. Serum samples from patients with PTC differed most significantly from those of patients with BND by the underexpression of a protein peak at 11 101 Da.
Conclusions:
This pilot study demonstrates that proteomic analysis of serum protein profiles distinguishes patients with PTC from patients with BND with a high degree of sensitivity and specificity. Further investigation into the clinical utility of this technology in PTC biomarker detection and surveillance is warranted.
