Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Fuel Cells01:23

Microbial Fuel Cells

Microbial fuel cells (MFCs) are bioelectrochemical devices that generate electricity by exploiting the metabolic processes of electrogenic bacteria. These systems provide a renewable energy source and serve as an innovative method for treating organic waste, such as wastewater.A typical MFC consists of two chambers: an anoxic (oxygen-free) compartment that houses the bacteria and an oxic (oxygen-rich) compartment that contains oxygen as the terminal electron acceptor. Many MFCs use proton...
Microbes and Methanogenesis01:26

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...
Microbial Wastewater Treatment01:30

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Microbial Mats01:25

Microbial Mats

Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
Microbial Bioremediation of Hydrocarbons01:26

Microbial Bioremediation of Hydrocarbons

Bioremediation is an environmentally sustainable process that employs living organisms—primarily microorganisms—to degrade or neutralize pollutants from contaminated environments. In oil spills and hydrocarbon pollution, bioremediation involves the use of hydrocarbon-degrading bacteria to transform toxic compounds into less harmful substances. This approach leverages natural microbial metabolic processes and is considered both cost-effective and ecologically favorable compared to physical or...
Bioremediation00:46

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The hidden potential of archaea in carbon and nitrogen cycling in agricultural soils: a review.

Frontiers in microbiology·2026
Same author

Electrochemical Upcycling of Shell Waste for Sustainable Nutrient Recovery from Wastewater.

Environmental science & technology·2025
Same author

Upscaled open-culture production of microbial flocculants from industrial wastewaters.

Trends in biotechnology·2025
Same author

Effect of substrate size reduction and periodic nutrient supplementation on biological wood oxidation.

Journal of environmental management·2024
Same author

Biological S<sup>0</sup> reduction at neutral and acidic conditions: Performance and microbial community shifts in a H<sub>2</sub>/CO<sub>2</sub>-fed bioreactor.

Water research·2024
Same author

Oxygen-to-ammonium-nitrogen ratio as an indicator for oxygen supply management in microoxic bioanodic ammonium oxidation.

Water research·2024

Related Experiment Video

Updated: Jul 7, 2026

Hydrogen Production and Utilization in a Membrane Reactor
10:00

Hydrogen Production and Utilization in a Membrane Reactor

Published on: March 10, 2023

Hydrogen production with a microbial biocathode.

René A Rozendal1, Adriaan W Jeremiasse, Hubertus V M Hamelers

  • 1Sub-Department of Environmental Technology, Wageningen University, Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.

Environmental Science & Technology
|February 21, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel microbial biocathode for efficient hydrogen production using a mixed culture of electrochemically active microorganisms. This breakthrough significantly enhances hydrogen yield and current density in microbial electrochemical technologies.

More Related Videos

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Hydrogen Production and Utilization in a Membrane Reactor
10:00

Hydrogen Production and Utilization in a Membrane Reactor

Published on: March 10, 2023

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

Area of Science:

  • Microbial electrochemistry
  • Biohydrogen production
  • Renewable energy technologies

Background:

  • Microbial fuel cells and microbial electrolysis cells are promising for sustainable energy generation.
  • Developing efficient biocathodes is crucial for optimizing hydrogen production in these systems.

Purpose of the Study:

  • To develop a novel microbial biocathode for enhanced hydrogen production.
  • To investigate the performance of a naturally selected mixed microbial culture for bioelectrochemical applications.

Main Methods:

  • A three-phase startup procedure was employed to convert an acetate- and hydrogen-oxidizing bioanode into a hydrogen-producing biocathode by reversing electrode polarity.
  • Performance was evaluated based on current density, potential, hydrogen production rate, and cathodic hydrogen efficiency.
  • Scanning electron microscopy was used to analyze biofilm formation on the electrode surface.

Main Results:

  • The developed microbial biocathode achieved a current density of -1.2 A/Nm2 at -0.7 V, 3.6 times higher than the control electrode (-0.3 A/m2).
  • Hydrogen production reached approximately 0.63 m3 H2/m3 cathode liquid volume/day with a 49% cathodic hydrogen efficiency, significantly outperforming the control (0.08 m3 H2/m3/day, 25% efficiency).
  • The biocathode's effluent successfully inoculated another cell, creating an identical hydrogen-producing biocathode, indicating robustness and scalability.

Conclusions:

  • A stable and highly efficient microbial biocathode for hydrogen production was successfully developed using a naturally selected mixed microbial culture.
  • The polarity reversal method is effective for transforming bioanodes into efficient biocathodes.
  • This technology holds significant potential for sustainable and cost-effective biohydrogen production.