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Updated: Jul 7, 2026
Detection and Isolation of Campylobacter spp. from Raw Meat
Published on: February 23, 2024
Quantitative isolation efficiency of O26, O103, O111, O145 and O157 STEC serotypes from artificially contaminated
B Possé1, L De Zutter, M Heyndrickx
1Faculty of Veterinart Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Hygiene and Technology, University of Ghent, Merelbeke, Belgium. Bjorn.posse@ugent.be
Aims:
A range of new differential and confirmation plating media for some non-O157 Shiga toxin producing Escherichia coli (STEC) serotypes (O26, O103, O111, O145) and both sorbitol-positive and -negative O157 were evaluated using artificially contaminated samples.
Methods And Results:
Dairy products (raw milk, cheese made from pasteurized milk and raw milk), meat (ground beef, fermented meat) and cattle faeces were artificially contaminated using clinical STEC strains. Isolation efficiency was 100%, 82.3%, 88.5%, 65.9%, 64.3% and 15.8%, respectively, for an inoculum size of
Conclusions:
Data presented in this paper indicated that the efficiency of the applied isolation method was dependent on sample-to-sample variation but not on the inoculum size.
Significance And Impact Of Study:
Data in this paper indicated that isolation of low levels of non-O157 and sorbitol-positive O157 STEC from food samples is possible.
