Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dermatological manifestations of hereditary fibrosing poikiloderma with tendon contractures, myopathy and pulmonary fibrosis (POIKTMP): a case series of 28 patients.

The British journal of dermatology·2019
Same author

Molecular phylogenetic position of podostemaceae, a marvelous aquatic flowering plant family.

Journal of plant research·2016
Same author

Reference intervals for immature platelet fraction and immature platelet count.

International journal of laboratory hematology·2014
Same author

Augmented reality intravenous injection simulator based 3D medical imaging for veterinary medicine.

Veterinary journal (London, England : 1997)·2012
Same author

Protein kinase C mRNA and protein expressions in hypobaric hypoxia-induced cardiac hypertrophy in rats.

Acta physiologica (Oxford, England)·2009
Same author

[Percutaneous cryosurgery for liver tumors].

Gan to kagaku ryoho. Cancer & chemotherapy·2001

Related Experiment Video

Updated: Jul 7, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Osteopontin expression in normal and hypobaric hypoxia-exposed rats.

M Uenoyama1, S Ogata, K Nakanishi

  • 1Division of Environmental Medicine, National Defense Medical College Research Institute, Tokorozawa, Japan.

Acta Physiologica (Oxford, England)
|February 21, 2008
PubMed
Summary

Hypobaric hypoxia exposure in rats showed decreased osteopontin (OPN) in plasma and adrenal glands, with transient increases in heart and lung. This suggests pulmonary hypertension may not significantly induce OPN without severe pressure overload.

More Related Videos

Measurement of Pulmonary Artery Pressure in Rats Using Right Heart Catheterization
07:21

Measurement of Pulmonary Artery Pressure in Rats Using Right Heart Catheterization

Published on: December 16, 2025

Related Experiment Videos

Last Updated: Jul 7, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Measurement of Pulmonary Artery Pressure in Rats Using Right Heart Catheterization
07:21

Measurement of Pulmonary Artery Pressure in Rats Using Right Heart Catheterization

Published on: December 16, 2025

Area of Science:

  • Cardiovascular Physiology
  • Altitude Medicine
  • Molecular Biology

Background:

  • Experimental pulmonary hypertension (PH) involves heart and pulmonary artery remodeling.
  • Osteopontin (OPN) is a key factor in cardiovascular remodeling due to pressure or volume overload.
  • The impact of hypobaric hypoxic environments (HHE) on OPN synthesis requires investigation.

Purpose of the Study:

  • To investigate the effects of HHE on the OPN synthesis system.
  • To determine OPN expression changes in response to simulated high altitude.

Main Methods:

  • 148 male Wistar rats were exposed to simulated 5500 m altitude for up to 21 days.
  • Plasma and tissue OPN levels were measured using various techniques.
  • Immunohistochemistry was used to assess OPN protein distribution.

Main Results:

  • Plasma and adrenal gland OPN levels decreased significantly by day 0.5 of HHE.
  • Right ventricle OPN mRNA and lung OPN protein increased transiently on days 1 and 5, respectively.
  • Changes in OPN synthesis did not correlate with the moderate increase in pulmonary arterial pressure (PAP).

Conclusions:

  • PH induced by HHE at 5500 m may cause minimal OPN induction in the heart and lungs.
  • Sustained OPN induction likely requires a more severe pulmonary arterial pressure overload.
  • OPN regulation in response to hypoxic PH is complex and depends on pressure severity.