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Updated: Jul 7, 2026

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
Published on: January 7, 2019
Focusing ultrasound in biological media
1Bio-Imaging Res. Inc., Lincolnshire, IL.
Abstract:
The authors present a formulation for calculating the field distributions for an ultrasonic imaging system when focusing in biological media. A biological medium is characterized in terms of its complex and frequency-dependent compressibility that, in turn, depends on the medium relaxation time and specific heat ratio. The analysis is based on a spatial/temporal transfer function formalism and can predict the effects of dispersion, frequency-dependent attenuation, aperture apodization and pulse shape. These effects can be observed on a ;snapshot' of the field in time and space. A number of simulations for a 3-MHz broadband system are presented. The results indicate that, aside from geometrical considerations (F/number), the pulse-width and the dispersive processes can play a major role in imaging performance.
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