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Updated: Jul 7, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Fluorescence readout of near-field photochromic memory
Abstract:
The fluorescence- and transmittance-detection readout methods of a near-field photochromic optical memory by a scanning-probe technique were studied theoretically. Shot noise, as the principal noise, was taken into account in an analysis of the signal-to-noise ratio (SNR). Under most conditions, fluorescence-detection readout yielded a better SNR than did transmittance-detection readout. For a transmittance change of 0.9-1.0 as a result of recording, a readout light power of approximately 100 nW, and a system bandwidth of 1 MHz, only the fluorescence-detection readout method, under the condition that the fluorescent layer of the medium have a fluorescence quantum yield greater than 0.4, can produce a sufficiently large SNR.

