Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical characteristics and prognostic analysis of acute erythroid leukemia].

Zhonghua nei ke za zhi·2026
Same author

[Clinical study of induction chemotherapy followed by allogeneic hematopoietic stem cell transplantation in the treatment of FLT3-ITD(+) acute myeloid leukemia with normal karyotype].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2023
Same author

Polymer-coated BiOCl nanosheets for safe and regioselective gastrointestinal X-ray imaging.

Journal of controlled release : official journal of the Controlled Release Society·2022
Same author

Prolonged QT dispersion in Subclinical Hypothyroid Females: A Study in University Teaching Hospital in Central Nepal.

Kathmandu University medical journal (KUMJ)·2020
Same author

[Research progress on exposure levels and toxic pathways of typical persistent organic pollutants in foods].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2019
Same author

Nuclear nanomedicine using Si nanoparticles as safe and effective carriers of <sup>188</sup>Re radionuclide for cancer therapy.

Scientific reports·2019

Related Experiment Video

Updated: Jul 7, 2026

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Two-photon-pumped frequency-upconverted blue lasing in coumarin dye solution.

G S He, R Signorini, P N Prasad

    Applied Optics
    |February 21, 2008
    PubMed
    Summary

    Two-photon-pumped blue lasing was achieved using Coumarin 500 dye. Researchers controlled lasing properties and achieved a 4.8% conversion efficiency for this frequency-upconverted laser technology.

    Area of Science:

    • Nonlinear optics
    • Laser physics
    • Materials science

    Background:

    • Two-photon-pumped (TPP) lasers offer unique frequency-upconversion capabilities.
    • Coumarin dyes are known for their fluorescence properties, making them candidates for TPP lasing.
    • Efficient blue light generation via TPP is of interest for various applications.

    Purpose of the Study:

    • To experimentally investigate two-photon-pumped frequency-upconverted blue lasing in Coumarin 500 dye solution.
    • To characterize the spectral, temporal, and spatial properties of the generated blue laser light.
    • To determine the output-input characteristics and conversion efficiency of the TPP lasing system.

    Main Methods:

    • Utilized ~800-nm laser pulses with ~5-ns duration to pump Coumarin 500 dye solution in chloroform.

    More Related Videos

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
    08:48

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

    Published on: November 22, 2019

    Generation and Coherent Control of Pulsed Quantum Frequency Combs
    06:42

    Generation and Coherent Control of Pulsed Quantum Frequency Combs

    Published on: June 8, 2018

    Related Experiment Videos

    Last Updated: Jul 7, 2026

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
    10:35

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

    Published on: May 29, 2018

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
    08:48

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

    Published on: November 22, 2019

    Generation and Coherent Control of Pulsed Quantum Frequency Combs
    06:42

    Generation and Coherent Control of Pulsed Quantum Frequency Combs

    Published on: June 8, 2018

  • Employed a 1-cm-long quartz cuvette to contain the dye solution for lasing.
  • Measured lasing wavelength, spectral structure, temporal dynamics, spatial profile, and output-input energy characteristics.
  • Investigated control of lasing properties by attaching an optical plate to the cuvette.
  • Main Results:

    • Achieved shortest lasing wavelength of approximately 479 nm (blue light).
    • Demonstrated control over spectral structure and longitudinal mode numbers by simple optical adjustments.
    • Measured a net conversion efficiency of approximately 4.8% from absorbed pump energy to blue laser output.

    Conclusions:

    • Two-photon-pumped blue lasing from Coumarin 500 dye solution is feasible and experimentally verified.
    • Lasing characteristics can be effectively tuned through simple optical modifications.
    • The achieved conversion efficiency indicates potential for practical applications of TPP blue lasers.