Proteomic studies reveal coordinated changes in T-cell expression patterns upon infection with human immunodeficiency
Jeffrey H Ringrose1, Rienk E Jeeninga, Ben Berkhout
1K1-262, Academic Medical Center, University of Amsterdam, Clinical Proteomics Group, Medical Biochemistry, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. d.speijer@amc.uva.nl
Journal of Virology
|February 22, 2008
Summary
Human immunodeficiency virus type 1 (HIV-1) infection alters T cell protein levels, causing metabolic shifts and influencing apoptosis and transport proteins. This proteomic study reveals key cellular changes during HIV infection.
Area of Science:
- Proteomics
- Immunology
- Virology
Background:
- Human immunodeficiency virus type 1 (HIV-1) infection significantly impacts host cell biology.
- Understanding cellular changes in T cells is crucial for HIV-1 pathogenesis research.
Purpose of the Study:
- To comprehensively analyze proteomic alterations in human T cells following HIV-1 infection.
- To identify specific proteins and pathways affected by HIV-1 at the cellular level.
Main Methods:
- Two-dimensional differential in-gel electrophoresis proteomic analysis.
- Identification and quantification of differentially expressed proteins in infected T cells.
Main Results:
- 15% of 2,000 detected protein spots showed differential expression at peak HIV-1 infection.
- 93 proteins were identified with altered abundance; 27 downregulated and 66 upregulated.
- Significant metabolic rerouting observed: downregulated glycolysis and enhanced mitochondrial oxidation.
- Proteins involved in apoptotic signaling and intracellular transport were notably affected.
Conclusions:
- HIV-1 infection induces widespread proteomic changes in T cells.
- Metabolic reprogramming, apoptosis modulation, and altered intracellular transport are key cellular responses to HIV-1.
- These findings provide insights into HIV-1's impact on cellular functions and potential therapeutic targets.
