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Related Concept Videos

Longitudinal Research02:20

Longitudinal Research

Sometimes we want to see how people change over time, as in studies of human development and lifespan. When we test the same group of individuals repeatedly over an extended period of time, we are conducting longitudinal research. Longitudinal research is a research design in which data-gathering is administered repeatedly over an extended period of time. For example, we may survey a group of individuals about their dietary habits at age 20, retest them a decade later at age 30, and then again...
Statistical Analysis System (SAS)01:14

Statistical Analysis System (SAS)

SAS, short for Statistical Analysis System, is a powerful data analysis, management, and visualization tool. Developed by the SAS Institute in the early 1970s, SAS has evolved into a comprehensive software suite used across various industries for statistical analysis, business intelligence, and predictive modeling.
Applications: SAS finds applications in numerous fields, including healthcare for clinical trial analysis, finance for risk assessment, marketing for customer data analysis, and...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Scalar Notation01:28

Scalar Notation

Scalar notation is a useful method for simplifying calculations involving vectors. When vectors are added or subtracted, their components can be added or subtracted separately using scalar notation. For instance, force, a vector quantity, can be broken down into its x and y components, called rectangular components, and then the magnitude and direction of these components can be determined using trigonometric functions.
Consider a man pulling a rope from a hook in the northeast direction. The...
Long-term Potentiation01:25

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Long-term Potentiation01:35

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.

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Related Experiment Video

Updated: Jul 7, 2026

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling (SAHM)
12:26

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling (SAHM)

Published on: October 11, 2016

SAGE and LongSAGE.

Annabeth Laursen Høgh1, Kåre Lehmann Nielsen

  • 1Department of Biochemistry, Chemistry and Environmental Engineering, University of Aalborg, Aalborg, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2008
PubMed
Summary

Serial analysis of gene expression (SAGE) offers high-throughput, quantitative gene analysis for discovering novel genes. While powerful and adaptable, its complex procedure limits routine laboratory application.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Serial analysis of gene expression (SAGE) is a high-throughput technique for global gene expression profiling.
  • It enables quantitative and simultaneous analysis of numerous transcripts, functioning as a digital method.
  • SAGE is valuable for identifying novel genes under specific conditions or in particular tissues.

Purpose of the Study:

  • To highlight the capabilities of SAGE in gene expression analysis.
  • To underscore its utility in diverse research fields like cancer and microbiology.
  • To discuss the practical challenges associated with its routine implementation.

Main Methods:

  • Utilizes a series of routine molecular biology procedures.
  • Relies on sequencing of gene tags for quantitative analysis.

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  • Applicable in principle to any laboratory setting.

    • Main Results:

    • SAGE provides sensitive and comprehensive gene expression data.
    • It has been successfully applied in cancer research and microbial studies.
    • The method's sensitivity is directly related to the number of tags sequenced.

    Conclusions:

    • SAGE is a potent tool for global gene expression analysis and novel gene discovery.
    • Despite its potential, the extensive number of steps complicates routine laboratory use.
    • Further optimization may be needed to enhance its practical accessibility.