Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An activity-resistance tradeoff constrains enzyme evolution.

bioRxiv : the preprint server for biology·2026

Same author

The role of genetic variation in shaping phenotypic responses to diet in aging Drosophila melanogaster.

Heredity·2025

Same author

The transmission ability in a population of elite tetraploid potatoes.

The plant genome·2025

Same author

The Role of Genetic Variation in Shaping Phenotypic Responses to Diet in Aging <i>Drosophila melanogaster</i>.

bioRxiv : the preprint server for biology·2025

Same author

Transcriptome analysis unravels the biocontrol mechanism of Serratia plymuthica A30 against potato soft rot caused by Dickeya solani.

PloS one·2024

Same author

To be or not to be tetraploid-the impact of marker ploidy on genomic prediction and GWAS of potato.

Frontiers in plant science·2024

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Cost-Efficient Transcriptomic-Based Drug Screening

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

Low-cost-medium throughput Sanger dideoxy sequencing.

Kåre Lehmann Nielsen¹

¹Department of Life Sciences, University of Aalborg, Aalborg, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|February 22, 2008

Summary

Serial analysis of gene expression (SAGE) relies on DNA sequencing. This study presents a cost-effective SAGE method using amplified inserts from concatemer clones for robust gene expression analysis.

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Serial analysis of gene expression (SAGE) is a powerful technique for analyzing gene expression profiles.
The primary cost driver for SAGE is DNA sequencing.
Affordable and robust sequencing systems are crucial for widespread SAGE adoption.

Purpose of the Study:

To develop a cost-effective and robust DNA sequencing system for Serial Analysis of Gene Expression (SAGE).
To reduce the overall cost associated with SAGE experiments.

Main Methods:

The study describes a novel SAGE system.
This system is based on sequencing amplified inserts derived from concatemer-containing clones.
This approach aims to improve sequencing efficiency and reduce costs.

More Related Videos

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Related Experiment Videos

Last Updated: Jul 7, 2026

Cost-Efficient Transcriptomic-Based Drug Screening

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Main Results:

The developed system provides a robust method for DNA sequencing applicable to SAGE.
The methodology is designed to be affordable, addressing a key limitation of traditional SAGE.
Sequencing amplified inserts of concatemer-containing clones offers a viable alternative for gene expression analysis.

Conclusions:

A robust and affordable sequencing system for SAGE has been successfully developed.
This advancement has the potential to lower the barrier to entry for gene expression studies using SAGE.
The described method offers a practical solution for cost-effective gene expression profiling.