Related Experiment Video
Updated: Jul 7, 2026
Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
Published on: May 2, 2025
Detection of GFP during nervous system development in Drosophila melanogaster
Karin Edoff1, James S Dods, Andrea H Brand
1Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, USA.
Abstract:
Using the vital marker GFP and its spectral variants, it is possible to visualize multiple proteins in individual cells and thereby monitor embryonic development on a cellular and molecular level. In the following chapter we describe how to prepare Drosophila embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.
