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Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Genomics02:02

Genomics

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Related Experiment Video

Updated: Jul 7, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

The EMBL Nucleotide Sequence and Genome Reviews Databases.

Peter Sterk1, Tamara Kulikova, Paul Kersey

  • 1EMBL-Outstation The European Bioinformatics Institute, Welcome Trust Genome Campus, Hinxton, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2008
PubMed
Summary

This chapter details the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database and Genome Reviews. These resources facilitate access to plant nucleotide sequences and genomic information for research.

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Novel Sequence Discovery by Subtractive Genomics
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Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Related Experiment Videos

Last Updated: Jul 7, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
09:37

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Published on: August 15, 2019

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • Nucleotide and protein sequence databases are crucial resources for biological and medical research.
  • Primary data archives store raw sequence information, while secondary databases offer curated and annotated views.

Purpose of the Study:

  • Introduce the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database as a primary archive.
  • Describe Genome Reviews as a secondary database for annotated genomic sequences.
  • Demonstrate data access, similarity searches, and information retrieval using Integr8, focusing on plant sequences.

Main Methods:

  • Utilizing the EMBL Nucleotide Sequence Database for primary data.
  • Accessing Genome Reviews for standardized, annotated genomic information.
  • Employing the Integr8 system for data exploration and analysis.

Main Results:

  • Demonstrated methods for accessing plant nucleotide sequences.
  • Illustrated sequence similarity search functionalities.
  • Showcased the retrieval of comprehensive genomic information via Integr8.

Conclusions:

  • The EMBL Nucleotide Sequence Database and Genome Reviews are vital for accessing and analyzing genomic data.
  • Integr8 provides a powerful platform for exploring plant genomic sequences and related information.