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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jul 7, 2026

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

Overview and introduction to clinical proteomics.

Young-Ki Paik1, Hoguen Kim, Eun-Young Lee

  • 1Department of Biochemistry, Yonsei Proteome Research Center & Biomedical Proteome Research Center, Seoul, Korea.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2008
PubMed
Summary

Clinical proteomics requires standardized procedures for handling specimens and reducing complexity to discover disease biomarkers. A shift towards using protein or peptide ensembles offers greater diagnostic efficiency than single biomarkers.

More Related Videos

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Related Experiment Videos

Last Updated: Jul 7, 2026

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Area of Science:

  • Clinical Proteomics
  • Biomarker Discovery
  • Translational Medicine

Background:

  • The advancement of clinical proteomics necessitates robust methods for identifying disease biomarkers.
  • Current challenges include standardizing specimen handling, reducing sample complexity, and enhancing detection of low-abundance proteins and peptides.

Purpose of the Study:

  • To address the challenges in clinical proteomics for disease biomarker discovery.
  • To highlight the need for standardized operating procedures and advanced techniques.

Main Methods:

  • Developing microdissection techniques for tissue specimens.
  • Implementing multidimensional fractionation for body fluid samples.
  • Integrating bioinformatics tools for data analysis.

Main Results:

  • Standardized procedures are crucial for reliable biomarker discovery.
  • Ensembles of proteins/peptides show greater diagnostic potential than single markers.
  • Advanced techniques enhance the detection capabilities in clinical proteomics.

Conclusions:

  • Establishing biorepository systems with well-defined clinical specimens is essential.
  • Microdissection, multidimensional fractionation, and bioinformatics are integral to clinical proteomics.
  • The paradigm is shifting towards using protein/peptide signatures for disease diagnosis.