Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Integrins01:10

Integrins

5.5K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
5.5K
Activation of Integrins01:15

Activation of Integrins

5.1K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
5.1K
Electron Affinity03:07

Electron Affinity

43.4K
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
43.4K
Affinity and Avidity01:41

Affinity and Avidity

39.2K
Overview
39.2K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.9K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.9K
Affinity Chromatography01:03

Affinity Chromatography

3.1K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic Profiling of Optic Nerves From SMOX-Deficient Mice Identifies Regulators of Neuroinflammation and Axonal Damage in Optic Neuritis.

Investigative ophthalmology & visual science·2026
Same author

Population-associated molecular variation in histologically normal breast tissue is context-dependent and associated with distinct transcriptional states.

bioRxiv : the preprint server for biology·2026
Same author

Integrated EWM-RSM optimization of wine-steaming enhances the anti-inflammatory activity of <i>Dendrobium huoshanense</i> polysaccharides.

Frontiers in chemistry·2026
Same author

Workload and personnel allocation in pharmacy departments of public hospitals in China: a current state study.

Scientific reports·2026
Same author

Harnessing endophytic fungi for sustainable agriculture: ecological roles, mechanisms, and future prospects.

Frontiers in microbiology·2026
Same author

Predicting mortality with machine learning & biomarkers: A cohort analysis from aerosolized β₂-agonist for treatment of acute lung injury (ALTA) trial.

Physiological reports·2026

Related Experiment Video

Updated: Feb 9, 2026

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of &#946;2 Integrin Activation
04:15

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

Published on: February 2, 2024

2.3K

Integrin affinity modulation in angiogenesis.

Ganapati H Mahabeleshwar1, Juhua Chen, Weiyi Feng

  • 1Department of Molecular Cardiology, Joseph J. Jacobs Center for Thrombosis and Vascular Biology, NB50, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

Cell Cycle (Georgetown, Tex.)
|February 22, 2008
PubMed
Summary
This summary is machine-generated.

Endothelial integrins regulate blood vessel growth. This study found that inhibiting beta(3) integrin significantly reduced vascular endothelial growth factor (VEGF)-induced angiogenesis, highlighting its critical role.

More Related Videos

In Vitro Model of Coronary Angiogenesis
08:03

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

8.5K
Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K

Related Experiment Videos

Last Updated: Feb 9, 2026

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of &#946;2 Integrin Activation
04:15

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

Published on: February 2, 2024

2.3K
In Vitro Model of Coronary Angiogenesis
08:03

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

8.5K
Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Angiogenesis Research

Background:

  • Integrins are transmembrane receptors crucial for cell adhesion and signaling.
  • Their role in pathological angiogenesis, particularly the specific functions of endothelial integrin beta subunits, is not fully elucidated.
  • Vascular Endothelial Growth Factor (VEGF) is a key stimulant of angiogenesis.

Purpose of the Study:

  • To investigate the regulatory functions of individual endothelial integrin beta subunits (beta(1), beta(3), beta(5)) in VEGF-induced angiogenic responses.
  • To determine the specific contribution of each beta subunit to endothelial cell (EC) adhesion, migration, and capillary growth.

Main Methods:

  • Inhibition of specific integrin beta subunit expression in endothelial cells.
  • Assessment of EC adhesion and migration on integrin-specific ligands.
  • Evaluation of capillary-like structure formation in response to VEGF stimulation.

Main Results:

  • Inhibition of beta(1), beta(3), or beta(5) integrin expression decreased EC adhesion and migration on their respective primary ligands.
  • No significant effects on the recognition of other ligands were observed after subunit inhibition.
  • While all tested subunit inhibitions impacted VEGF-stimulated capillary growth, the absence of beta(3) integrin demonstrated the most pronounced inhibitory effect.

Conclusions:

  • Individual endothelial integrin beta subunits play distinct roles in mediating EC responses to VEGF.
  • Beta(3) integrin is a critical regulator of VEGF-induced angiogenesis, showing a more significant inhibitory effect upon its loss compared to beta(1) or beta(5).
  • These findings contribute to a better understanding of integrin function in angiogenesis and may inform therapeutic strategies targeting pathological vessel formation.