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Gold nanohole array substrates as immunobiosensors.

John C Sharpe1, John S Mitchell, Ling Lin

  • 1The Horticulture and Food Research Institute of New Zealand, East Street, Private Bag 3123, Hamilton 3240, New Zealand. jsharpe@hortresearch.co.nz

Analytical Chemistry
|February 22, 2008
PubMed
Summary
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Gold nanohole arrays detect monoclonal antibody binding via transmission peak shifts. This sensitive, regenerable method enhances detection using secondary antibody-gold nanoparticle conjugates for biomolecule sensing.

Area of Science:

  • Nanotechnology
  • Biosensing
  • Immunotechnology

Background:

  • Gold nanohole arrays offer unique optical properties for sensing applications.
  • Cortisol detection is crucial for diagnosing various medical conditions.
  • Existing immunoassay methods may lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a sensitive and regenerable biosensing platform using gold nanohole arrays.
  • To detect monoclonal antibody binding to a functionalized cortisol derivative.
  • To enhance detection sensitivity using gold nanoparticle conjugates.

Main Methods:

  • Functionalization of a gold nanohole array with a cortisol thiol derivative.
  • Detection of monoclonal antibody binding via shifts in the 1060 nm transmission peak.

Related Experiment Videos

  • Application of a secondary antibody-gold nanoparticle conjugate to enhance signal.

    • Main Results:

    • A sensitive shift in the transmission peak was observed upon antibody binding.
    • Detection sensitivity was enhanced threefold by the secondary antibody-gold nanoparticle conjugate.
    • The nanohole array demonstrated a regenerable response.

    Conclusions:

    • Gold nanohole arrays provide a sensitive platform for immunobiosensing.
    • The developed method offers enhanced detection for biomolecules like cortisol.
    • This approach has potential applications in diagnosing stress-related disorders and other conditions.