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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jul 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Gold nanohole array substrates as immunobiosensors.

John C Sharpe1, John S Mitchell, Ling Lin

  • 1The Horticulture and Food Research Institute of New Zealand, East Street, Private Bag 3123, Hamilton 3240, New Zealand. jsharpe@hortresearch.co.nz

Analytical Chemistry
|February 22, 2008
PubMed
Summary
This summary is machine-generated.

Gold nanohole arrays detect monoclonal antibody binding via transmission peak shifts. This sensitive, regenerable method enhances detection using secondary antibody-gold nanoparticle conjugates for biomolecule sensing.

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Last Updated: Jul 7, 2026

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07:30

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Published on: March 7, 2018

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

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Published on: November 10, 2016

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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Area of Science:

  • Nanotechnology
  • Biosensing
  • Immunotechnology

Background:

  • Gold nanohole arrays offer unique optical properties for sensing applications.
  • Cortisol detection is crucial for diagnosing various medical conditions.
  • Existing immunoassay methods may lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a sensitive and regenerable biosensing platform using gold nanohole arrays.
  • To detect monoclonal antibody binding to a functionalized cortisol derivative.
  • To enhance detection sensitivity using gold nanoparticle conjugates.

Main Methods:

  • Functionalization of a gold nanohole array with a cortisol thiol derivative.
  • Detection of monoclonal antibody binding via shifts in the 1060 nm transmission peak.
  • Application of a secondary antibody-gold nanoparticle conjugate to enhance signal.

Main Results:

  • A sensitive shift in the transmission peak was observed upon antibody binding.
  • Detection sensitivity was enhanced threefold by the secondary antibody-gold nanoparticle conjugate.
  • The nanohole array demonstrated a regenerable response.

Conclusions:

  • Gold nanohole arrays provide a sensitive platform for immunobiosensing.
  • The developed method offers enhanced detection for biomolecules like cortisol.
  • This approach has potential applications in diagnosing stress-related disorders and other conditions.