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Updated: Jul 7, 2026
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Published on: June 8, 2019
[Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence
Zhao-Xia Zhang1, Li-Zhi Cao, Qiong Huang
1Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).
Methods:
The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.
Results:
CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).
Conclusions:
CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.
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