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Updated: Jul 7, 2026
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
Published on: April 20, 2021
Analysis of B cell selection in the germinal center reaction during a T-dependent antibody response at a single cell
Takahiro Okazawa1, Masaki Magari, Takafumi Kimoto
1Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
The quasimonoclonal mouse is useful to examine B cell selection during T-dependent antibody (Ab) responses because of its limited B cell populations mainly expressing the knockin 17.2.25 V(H)-encoded H chain (V(H)T) paired with the lambda1 or lambda2 L chain. It has been reported that both two V(H)T/lambda1 and V(H)T/lambda2 B cell populations responded to a T-dependent antigen conjugated with a hapten p-nitrophenylacetyl (pNP), but only V(H)T/lambda2 B cells differentiated to secrete high affinity anti-pNP IgG Abs by acquiring a critical mutation (T313A) in the V(H)T. The V(H)T/lambda2 B cells may be more potent in migrating to the germinal centers (GCs) due to about 50-fold higher affinity for pNP than V(H)T/lambda1 B cells. Here, to uncover how V(H)T/lambda2 B cells were preferentially recruited for affinity maturation during the anti-pNP Ab response, we examined the L chain usage and mutation frequency of V(H)T(+) GC B cells at a single cell level. V(H)T/lambda2 B cells bearing the unmutated V(H)T gene were found in the GCs more frequently than V(H)T/lambda1 and mutated V(H)T/lambda2 counterparts in an early phase of the Ab response. In the course of the GC reaction, the number of V(H)T/lambda2 B cells that mutated their V(H)T genes preferentially expanded, and finally V(H)T/lambda2 B cells bearing the T313A mutation occupied V(H)T(+) GC B cell population. Thus, it is suggested that B cells with a higher affinity were selected not only for entry to the GCs but also in the affinity maturation process during a T-dependent Ab response.
The quasimonoclonal mouse is useful to examine B cell selection during T-dependent antibody (Ab) responses because of its limited B cell populations mainly expressing the knockin 17.2.25 V(H)-encoded H chain (V(H)T) paired with the lambda1 or lambda2 L chain. It has been reported that both two V(H)T/lambda1 and V(H)T/lambda2 B cell populations responded to a T-dependent antigen conjugated with a hapten p-nitrophenylacetyl (pNP), but only V(H)T/lambda2 B cells differentiated to secrete high affinity anti-pNP IgG Abs by acquiring a critical mutation (T313A) in the V(H)T. The V(H)T/lambda2 B cells may be more potent in migrating to the germinal centers (GCs) due to about 50-fold higher affinity for pNP than V(H)T/lambda1 B cells. Here, to uncover how V(H)T/lambda2 B cells were preferentially recruited for affinity maturation during the anti-pNP Ab response, we examined the L chain usage and mutation frequency of V(H)T(+) GC B cells at a single cell level. V(H)T/lambda2 B cells bearing the unmutated V(H)T gene were found in the GCs more frequently than V(H)T/lambda1 and mutated V(H)T/lambda2 counterparts in an early phase of the Ab response. In the course of the GC reaction, the number of V(H)T/lambda2 B cells that mutated their V(H)T genes preferentially expanded, and finally V(H)T/lambda2 B cells bearing the T313A mutation occupied V(H)T(+) GC B cell population. Thus, it is suggested that B cells with a higher affinity were selected not only for entry to the GCs but also in the affinity maturation process during a T-dependent Ab response.
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