Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Dissection of the Endolymphatic Sac from Mice

Dissection of the Endolymphatic Sac from Mice

Published on: March 29, 2021

A mouse model with postnatal endolymphatic hydrops and hearing loss.

Cliff A Megerian¹, Maroun T Semaan, Saba Aftab

¹Department of Otolaryngology-Head and Neck Surgery, Case Western Reserve University, University Hospitals-Case Medical Center, 11100 Euclid Avenue, Cleveland, OH 44106, USA.

Hearing Research

|February 22, 2008

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clarin-1 mRNA expression in the central auditory system of Fischer Brown Norway rats.

Hearing research·2026

Same author

Dietary sodium propionate enhances growth performance, digestive enzymes, haematology and immunity in Wallago attu.

Journal of fish biology·2026

Same author

Implanted injection port for routine drug delivery to the middle ear in mice.

PloS one·2026

Same author

TNFAIP8L2 as a dual regulator of mTORC1 signaling: Implications for therapy in presbycusis.

Molecular therapy : the journal of the American Society of Gene Therapy·2025

Same author

Long-Lasting Auditory and Vestibular Recovery Following Gene Replacement Therapy in a Novel Usher Syndrome Type 1c Mouse Model.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Dietary inclusion of black soldier fly (Hermetia illucens) larvae meal, with exogenous protease supplementation, in practical diets for striped catfish (Pangasius hypophthalmus, Sauvage 1878).

PloS one·2024

The Phex Hyp-Duk/Y mouse model exhibits progressive hearing loss and endolymphatic hydrops (ELH) postnatally, with neuronal degeneration preceding structural changes. This model develops ELH without endolymphatic duct obstruction, offering new insights into ELH development.

Area of Science:

Otolaryngology
Genetics
Neuroscience

Background:

Endolymphatic hydrops (ELH), hearing loss, and neuronal degeneration are key features of Meniere's disease (MD).
The precise sequence and interrelationship of these pathological events remain unclear.
A spontaneous animal model is needed to investigate these conditions.

Purpose of the Study:

To characterize the inner ear phenotype in Phex Hyp-Duk/Y mice.
To test if Phex Hyp-Duk/Y mice develop postnatal hearing loss and ELH.
To determine if ELH in these mice is linked to endolymphatic duct (ED) obstruction.

Main Methods:

Auditory brainstem response (ABR) recordings at multiple time points.
Histological analysis of temporal bones from Phex Hyp-Duk/Y mice.

More Related Videos

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

Published on: June 4, 2020

Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct

Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct

Published on: January 22, 2010

Related Experiment Videos

Last Updated: Jul 7, 2026

Dissection of the Endolymphatic Sac from Mice

Dissection of the Endolymphatic Sac from Mice

Published on: March 29, 2021

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

Published on: June 4, 2020

Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct

Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct

Published on: January 22, 2010

Comparison of mutant mice with wild-type controls.

Main Results:

Phex Hyp-Duk/Y mice exhibit adult-onset, progressive, asymmetric hearing loss followed by ELH.
Functional degeneration, specifically spiral ganglion cell loss, precedes structural degeneration.
ELH develops in these mice without obstruction of the endolymphatic duct (ED).

Conclusions:

The Phex Hyp-Duk/Y mouse is a valuable model for studying hearing loss and ELH.
Neuronal degeneration is a primary event in the pathogenesis of hearing loss and ELH in this model.
ELH can develop independently of ED obstruction, suggesting alternative mechanisms are involved.