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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jul 7, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Scanning tomographic acoustic microscopy.

R Y Chiao1, H Lee

  • 1Gen. Electr. Corp. Res. and Dev. Center, Schenectady, NY.

IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

The scanning tomographic acoustic microscope (STAM) offers improved resolution over the scanning laser acoustic microscope (SLAM). This study demonstrates STAM

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Area of Science:

  • Acoustic microscopy
  • Tomography
  • Wavefield imaging

Background:

  • Scanning laser acoustic microscopy (SLAM) has limitations in resolution.
  • Tomographic principles offer potential for enhanced imaging.
  • Early work proposed scanning tomographic acoustic microscopy (STAM) in 1982.

Purpose of the Study:

  • To implement and demonstrate the scanning tomographic acoustic microscope (STAM).
  • To showcase the superior resolution capabilities of STAM compared to SLAM.
  • To validate STAM for imaging multiple-layer specimens.

Main Methods:

  • Modification of SLAM with a quadrature detector to acquire amplitude and phase information.
  • Utilizing tomographic projections with varying incident waves.
  • Applying the "back-and-forth" propagation algorithm for tomographic reconstruction.

Main Results:

  • Successful experimental acquisition of tomographic projections.
  • Generation of the first STAM reconstructions.
  • Demonstration of enhanced resolution in STAM compared to SLAM.
  • Experimental reconstruction of multi-layer specimens achieved.

Conclusions:

  • STAM implementation is feasible and effective.
  • STAM provides superior resolution for acoustic microscopy.
  • STAM is a viable technique for detailed imaging of complex specimens.