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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Jul 7, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Lens design for acoustic microscopy.

C H Chou¹, B T Khuri-Yakub, G S Kino

¹High Energy Phys. Lab., Stanford Univ., CA.

IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control

|January 1, 1988

Summary

This summary is machine-generated.

Acoustic microscope lens design varies for surface versus subsurface imaging. Different applications and materials necessitate specific lenses for optimal performance, impacting imaging capabilities.

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Area of Science:

Acoustic microscopy
Materials science
Optical engineering

Background:

Acoustic microscopes utilize lenses for high-resolution imaging.
Lens design is critical for effective surface wave excitation and imaging.
Understanding design criteria is essential for optimizing acoustic microscopy performance.

Purpose of the Study:

To examine acoustic microscope lens design criteria based on application.
To differentiate design needs for surface and subsurface examination.
To present a method for evaluating acoustic microscope performance.

Main Methods:

Analysis of factors influencing lens design, including critical angle, illumination, and surface wave leak rate.
Theoretical and experimental evaluation of acoustic microscope performance in the time domain.

Main Results:

Lens design criteria differ significantly for surface and subsurface acoustic microscopy.
Optimal imaging performance requires application- and material-specific lenses.
A time-domain evaluation formalism for acoustic microscopes was developed.

Conclusions:

Acoustic microscope lens design is application-dependent.
Material properties and examination type dictate lens requirements.
The presented formalism aids in performance assessment of acoustic microscopes.