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[Flocculent genomic clone and flocculating mechanism analysis].

Yu-guang Chang¹, Fang Ma, Jing-bo Guo

¹School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. changyuguang@yahoo.com.cn

Huan Jing Ke Xue= Huanjing Kexue

|February 23, 2008

Summary

Researchers isolated Bacillus sp. F2, a potent bioflocculant. They developed a genomic library and identified clone FC2, enhancing flocculation efficiency to 90% for effective kaolin particle removal.

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Area of Science:

Microbiology
Biotechnology
Environmental Science

Background:

Bacillus sp. F2 exhibits significant flocculation ability (84%).
Developing effective bioflocculants is crucial for environmental remediation.
Genomic libraries aid in identifying and enhancing microbial functions.

Purpose of the Study:

To construct a flocculent genomic library from Bacillus sp. F2.
To identify and characterize a recombinant strain with improved flocculation properties.
To investigate the microtopography and flocculation mechanism of the engineered strain.

Main Methods:

Genomic DNA extraction, partial digestion (Sau3AI), and ligation into PUC19DNA.
Transformation into competent E. coli JM109 cells and screening via white-blue spot assay.

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Flocculation tests, Atomic Force Microscopy (AFM), and Zeta-potential analysis.

Main Results:

A flocculent genomic library was successfully constructed.
Positive clone FC2 demonstrated enhanced flocculation efficiency (90%).
AFM revealed FC2 promotes compact, spherical floc structures with high adsorption capacity for kaolin particles.

Conclusions:

FC2 effectively inherits and enhances the flocculation capability of Bacillus sp. F2.
The bioflocculant exhibits significant potential for pollution removal, particularly in kaolin suspensions.
Zeta-potential analysis provides insights into the electrostatic interactions governing bioflocculation mechanisms.