Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Investigating fixative-induced changes in RNA quality and utility by microarray analysis.

Melissa L Cox1, Susan M Eddy, Zachary S Stewart

  • 1Drug Safety, Pfizer Global Research and Development, Ann Arbor, MI, USA.

Experimental and Molecular Pathology
|February 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting the NLRP3 inflammasome reduces inflammation in hidradenitis suppurativa skin.

The British journal of dermatology·2023
Same author

Cytokine induced 3-D organotypic psoriasis skin model demonstrates distinct roles for NF-κB and JAK pathways in disease pathophysiology.

Experimental dermatology·2022
Same author

Besnoitiosis in a miniature donkey.

Veterinary dermatology·2021
Same author

Histologic progression of acne inversa/hidradenitis suppurativa: Implications for future investigations and therapeutic intervention.

Experimental dermatology·2020
Same author

Zucker Lean Rats With Hepatic Steatosis Recapitulate Asymptomatic Metabolic Syndrome and Exhibit Greater Sensitivity to Drug-Induced Liver Injury Compared With Standard Nonclinical Sprague-Dawley Rat Model.

Toxicologic pathology·2020
Same author

Great Lakes Biorepository Research Network's Annual Biobanking Symposium: A Focus on Precision Medicine.

Biopreservation and biobanking·2019
Same journal

Bacteriological profile of secondary infections following snakebite envenomation's: A systematic literature review.

Experimental and molecular pathology·2026
Same journal

3D virtual histopathology of human breast tissues using an X-ray grating interferometry micro CT system.

Experimental and molecular pathology·2026
Same journal

Pan-cancer analysis identifies GPRIN1 as a prognostic biomarker and promoter of cell proliferation in pancreatic cancer.

Experimental and molecular pathology·2026
Same journal

MicroRNAs and predicted targets in the switch from monolayered to spheroids of cholangiocarcinoma cells.

Experimental and molecular pathology·2026
Same journal

Tumor-infiltrating CD8<sup>+</sup>Ki-67<sup>+</sup> and CD8<sup>+</sup>LAG-3<sup>+</sup> T cells are associated with improved patient survival in retroperitoneal dedifferentiated liposarcomas and leiomyosarcomas.

Experimental and molecular pathology·2026
Same journal

Citalopram enhances cisplatin-induced cytotoxicity in T24 bladder cancer cells: An in vitro study.

Experimental and molecular pathology·2026
See all related articles

Choosing the right tissue fixative is crucial for RNA quality. Modified methacarn best preserves RNA for microarray analysis, while 10% neutral buffered formalin significantly degrades it.

Area of Science:

  • Molecular Biology
  • Histology
  • Biochemistry

Background:

  • Preserving RNA quality is essential for accurate gene expression analysis.
  • Different tissue fixatives impact nucleic acid integrity differently.
  • Microarray analysis requires high-quality RNA for reliable results.

Purpose of the Study:

  • To evaluate the impact of three common fixatives (10% neutral buffered formalin, modified methacarn, 70% ethanol) on RNA quality.
  • To compare RNA integrity from fixed tissues with OCT-embedded and flash-frozen controls.
  • To determine the suitability of RNA from fixed tissues for microarray analysis.

Main Methods:

  • RNA isolation from rat livers fixed in different agents and stored for 1 month and 1 year.
  • Whole genome microarray analysis of isolated RNA.

More Related Videos

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis
09:21

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis

Published on: April 17, 2026

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

Related Experiment Videos

Last Updated: Jul 7, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis
09:21

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis

Published on: April 17, 2026

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

  • Comparison of RNA information content across different fixation methods and storage durations.

    • Main Results:

    • OCT-embedded tissue showed a minor RNA information loss (up to 5%) compared to snap-frozen controls.
    • Modified methacarn fixation resulted in the least RNA information loss (approx. 10%).
    • 70% ethanol and 10% neutral buffered formalin caused significant RNA degradation (approx. 25% and 80% loss, respectively).

    Conclusions:

    • Modified methacarn is the preferred fixative for preserving RNA quality for microarray analysis when morphology is critical.
    • RNA from 10% neutral buffered formalin-fixed tissue is unreliable for microarrays and better suited for techniques like quantitative RT-PCR.
    • Fixative choice significantly influences RNA integrity and downstream molecular applications.