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Persistent herpes simplex virus infections established in two Burkitt lymphoma derived cell lines
The Journal of General Virology
|July 1, 1976
Summary
Persistent Epstein-Barr virus (EBV) infected cells can establish long-term infections with herpes simplex virus type 1 (HSV-1). This study shows that HSV-1 can persist in Burkitt lymphoma cells without external support.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Epstein-Barr virus (EBV) producer P3HR-I cells were examined for persistent infection with herpes simplex virus type 1 (HSV-1).
- Previous studies suggested limited active HSV-1 replication despite antigen presence in persistently infected cells.
Purpose of the Study:
- To determine if persistent herpes simplex virus (HSV) infections could be established in Burkitt lymphoma-derived cell lines.
- To investigate the generalizability of HSV persistent infections across different HSV strains and cell types.
Main Methods:
- Infection of P3HR-I and Raji cell lines with multiple strains of HSV-1 and HSV-2.
- Analysis of viral DNA incorporation using 14C-thymidine labeling and cesium chloride gradients.
- Hybridization techniques to confirm HSV-1 DNA presence.
Main Results:
- Persistent HSV-1 infections were established in P3HR-I cells with all tested HSV-1 strains, but not with HSV-2.
- Raji cells, which are EBV non-producers, established persistent infections with most HSV-1 strains and both HSV-2 strains.
- These persistent infections were maintained without the need for external support.
Conclusions:
- Burkitt lymphoma-derived cells can support persistent infections by various strains of HSV-1 and, in some cases, HSV-2.
- The establishment of persistent HSV infection in these cell lines is a general phenomenon, independent of EBV status.
- HSV persistent infections in these cell lines do not require external support for maintenance.