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Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

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Related Experiment Video

Updated: Jul 7, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

Wnt and hedgehog signaling pathways in bone development.

Timothy F Day¹, Yingzi Yang

¹Genetic Disease Research Branch, National Human Genome Research Institute, Building 49, Room 4A68, 49 Convent Drive, MSC 4472, Bethesda, MD 20892, USA.

The Journal of Bone and Joint Surgery. American Volume

|March 20, 2008

Summary

This summary is machine-generated.

Wnt/beta-catenin signaling guides skeletal development by controlling cell differentiation. Indian hedgehog (Ihh) signaling integrates with Wnt signaling, suggesting therapeutic targets for bone formation and fracture repair.

More Related Videos

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Related Experiment Videos

Last Updated: Jul 7, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Area of Science:

Skeletal Biology and Development
Cell Signaling Pathways
Molecular Regulatory Networks

Background:

Cell-cell signaling is crucial for vertebrate embryonic development, controlling cell proliferation, differentiation, and survival.
These signaling pathways are also vital for adult tissue regeneration and repair, including skeletal tissues.
Wnt/beta-catenin signaling is a key pathway influencing osteoblast and chondrocyte differentiation in skeletal development.

Purpose of the Study:

To investigate the role of Wnt/beta-catenin signaling in skeletal development and its integration with Indian hedgehog (Ihh) signaling.
To elucidate the molecular mechanisms governing osteoblast and chondrocyte differentiation.
To identify potential therapeutic strategies for enhancing bone formation and fracture repair.

Main Methods:

Genetic manipulation in mouse embryos to ablate or upregulate beta-catenin.
Analysis of intramembranous and endochondral ossification processes.
Investigating the interplay between Wnt/beta-catenin and Ihh signaling pathways.

Main Results:

Genetic ablation of beta-catenin led to increased chondrocyte formation at the expense of osteoblasts.
Upregulation of Wnt signaling suppressed chondrocyte formation and promoted ossification.
Wnt signaling acts downstream of Ihh signaling, promoting osteoblast maturation during endochondral bone formation.

Conclusions:

Wnt/beta-catenin signaling precisely controls the balance between osteoblast and chondrocyte differentiation.
Integration of Ihh and Wnt signaling pathways is critical for skeletal development.
Targeting Ihh and Wnt signaling at specific stages may enhance bone formation and fracture healing.