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Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphatic Vessels and Lymph Transport01:16

Lymphatic Vessels and Lymph Transport

Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular spaces.
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

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Related Experiment Video

Updated: Jul 7, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Human lymphatic endothelial cells express multiple functional TLRs.

Amarendra Pegu1, Shulin Qin, Beth A Fallert Junecko

  • 1Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|February 23, 2008
PubMed
Summary
This summary is machine-generated.

Lymphatic endothelial cells (LECs) express Toll-like receptors (TLRs) 1-6 and 9, responding to various ligands by producing inflammatory molecules. This reveals their role in immune responses and dendritic cell (DC) interactions within lymphatic vessels.

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Published on: January 7, 2019

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05:52

Murine Dermal Lymphatic Endothelial Cell Isolation

Published on: July 21, 2023

Area of Science:

  • Immunology
  • Cell Biology
  • Vascular Biology

Background:

  • Lymphatic endothelial cells (LECs) are crucial for fluid drainage and immune cell transport to lymph nodes.
  • Toll-like receptors (TLRs) are key in pathogen recognition and immune activation.
  • The expression and function of TLRs in LECs are not well understood.

Purpose of the Study:

  • To comprehensively investigate the expression of Toll-like receptors (TLRs) 1-10 in human lymphatic endothelial cells (LECs).
  • To determine the functional response of LECs to various TLR ligands.
  • To elucidate the role of LECs in immune surveillance and dendritic cell (DC) migration.

Main Methods:

  • Analysis of TLR1-10 mRNA and protein expression in primary human dermal and lung LECs, and immortalized LECs.
  • Stimulation of LECs with a panel of ligands for TLR1-9.
  • Measurement of inflammatory chemokines, cytokines, and adhesion molecule expression using real-time RT-PCR, ELISA, and flow cytometry.

Main Results:

  • Human LECs express mRNA and protein for TLR1-6 and TLR9.
  • LECs respond to most tested TLR ligands by upregulating inflammatory mediators and adhesion molecules.
  • This indicates functional TLR expression in lymphatic endothelium.

Conclusions:

  • LECs possess a functional TLR repertoire, enabling them to sense pathogens and contribute to immune responses.
  • These findings highlight the lymphatic vasculature's role in shaping the immune microenvironment.
  • Understanding LEC TLRs offers insights into DC migration and potential vaccine adjuvant strategies.