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Interaction between Pazufloxacin and DNA mediated by copper(II) ions
Guoliang Zhang1, Xuchun Fu, Qingwang Liu
1College of Science, Zhejiang University, Hangzhou, People's Republic of China.
Abstract:
The influence of copper(II) ions on the binding of Pazufloxacin (PFLX), which is a forth generation quinolone antimicrobial agent, to calf thymus DNA (CTDNA) has been investigated by fluorescence, absorption spectroscopy and viscosity measurement. The results show that PFLX and Cu(2+) can form binary complex with mole ratio 2:1, PFLX and CTDNA can form complex mediated by Cu(2+). A ternary system of PFLX-Cu(2+)-DNA were proposed. The experimental results show that the PFLX binds to CTDNA by two kinds of binding modes, intercalative mode and groove binding mode.
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