Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is clonal haematopoiesis the missing link between lupus and cardiovascular disease?

Rheumatology (Oxford, England)·2026
Same author

Endothelial dysfunction in APS: advancing pathophysiological understanding to improve management.

Current opinion in immunology·2026
Same author

Revisiting antiphospholipid syndrome: A thrombo-inflammatory disorder beyond clotting.

Blood reviews·2026
Same author

Von Willebrand factor deficiency impairs angiogenesis via angiopoietin-2: relevance for gut angiodysplasia.

Blood·2026
Same author

Similar Medicolegal Patterns Emerge for Cranial Surgery Cases Globally.

World neurosurgery·2025
Same author

Comparative Viability and Functionality of Bio-Jetted and Threaded Human Umbilical Vein Endothelial Cells.

Small science·2025

Related Experiment Video

Updated: Jul 7, 2026

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

Gene expression studies on bio-electrosprayed primary cardiac myocytes.

Seán P Barry1, Suwan N Jayasinghe, Charis Pericleous

  • 1Medical Molecular Biology Unit, Institute of Child Health, University College London, London, UK.

Biotechnology Journal
|February 23, 2008
PubMed
Summary

Bio-electrospraying (BES) shows no negative impact on cardiac myocyte gene expression or endothelial cell activation. This cell engineering protocol is safe for cardiac tissue engineering applications.

More Related Videos

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart
05:06

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
11:13

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Published on: May 12, 2017

Related Experiment Videos

Last Updated: Jul 7, 2026

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart
05:06

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
11:13

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Published on: May 12, 2017

Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Cardiovascular Science

Background:

  • Cardiovascular diseases are a leading cause of mortality.
  • Ex vivo cardiac tissue development offers therapeutic potential.
  • Bio-electrospraying (BES) is a novel direct cell engineering protocol for regenerative medicine.

Purpose of the Study:

  • To evaluate the physiological effects of BES on cardiac myocytes and endothelial cells.
  • To determine if BES impacts global gene expression in cardiac myocytes.
  • To assess endothelial cell activation following BES treatment.

Main Methods:

  • Genome-wide microarray analysis was used to assess gene expression in cardiac myocytes.
  • Primary cardiac myocytes and endothelial cells were subjected to the BES protocol.
  • Cellular physiology, stress pathways, and DNA damage were previously assessed.

Main Results:

  • BES does not induce cell death, stress pathways, or DNA damage in cardiac myocytes.
  • Genome-wide microarray analysis revealed no negative effects on global gene expression in cardiac myocytes.
  • BES treatment did not lead to endothelial cell activation.

Conclusions:

  • BES demonstrates minimal adverse effects on the physiology of cardiac myocytes and endothelial cells.
  • These findings support the advancement of BES for cardiac tissue engineering.
  • BES is a promising technology for cardiac regenerative and therapeutic medicine.