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Updated: Jul 7, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display

Myung-Shin Lee1, Ji Chul Lee, Cha Yong Choi

  • 1Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon, South Korea.

Hybridoma (2005)
|February 26, 2008
PubMed

A monoclonal antibody to the light chain of botulinum neurotoxin type B (BoNT/B) was generated and its protective activity was evaluated in vivo. A chimeric rabbit/human Fab library was generated using bone marrow and spleen cDNAs of rabbits immunized with the BoNT/B light chain, and three monoclonal antibodies specific to the catalytic domain of BoNT/B were isolated. One of these clones, BCXRH1, was specific to a conformation-dependent epitope, and partially neutralized the BoNT/B complex in vivo.

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