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Updated: Jul 7, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
Generation and identification of natural monoclonal antibodies against low-density lipoprotein
Xuyang Feng1, Ruifen Xu, Yan Gao
1Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Low-density lipoprotein (LDL) is a major trigger of atherosclerotic lesions. Here we report the production of natural mouse IgM monoclonal antibodies (MAbs) against LDL with high specificity and activity, which could provide a potential value for research on lipid metabolism and atherosclerosis progression. BALB/c mice were raised in specific pathogen-free conditions and fed a high cholesterin diet; the splenocytes from these mice were directly fused with Sp2/0 myeloma cells using standard hybridoma production techniques. Resulting hybridomas producing anti-LDL antibodies were screened by enzyme-linked immunosorbent assay (ELISA) and isotyped. As a result, two hybridoma cell lines, named 5G8 and 2H7, were developed, which could secrete anti-LDL MAbs stably. Both of them belonged to the IgM subclass. The specificity of the MAb was determined based on its activity in Western blot and immunoprecipitation analysis.
Low-density lipoprotein (LDL) is a major trigger of atherosclerotic lesions. Here we report the production of natural mouse IgM monoclonal antibodies (MAbs) against LDL with high specificity and activity, which could provide a potential value for research on lipid metabolism and atherosclerosis progression. BALB/c mice were raised in specific pathogen-free conditions and fed a high cholesterin diet; the splenocytes from these mice were directly fused with Sp2/0 myeloma cells using standard hybridoma production techniques. Resulting hybridomas producing anti-LDL antibodies were screened by enzyme-linked immunosorbent assay (ELISA) and isotyped. As a result, two hybridoma cell lines, named 5G8 and 2H7, were developed, which could secrete anti-LDL MAbs stably. Both of them belonged to the IgM subclass. The specificity of the MAb was determined based on its activity in Western blot and immunoprecipitation analysis.
