Ontogeny of the response of mouse thymocytes to interleukin 1 and interleukin 2

In the present report we demonstrate that the in vitro proliferative response of the newborn thymocytes to interleukin (IL) 1 and IL 2, which is remarkably stronger than the adult thymocyte response, is associated with a considerable increase of CD4-CD8- cells expressing a gamma/delta T cell receptor (TcR). By polymerase chain reaction analysis we show that the V gamma gene segment usage in the adult and newborn responding cells reflects the developmentally regulated expression of the V gamma gene segments, suggesting that the increase in TcR gamma/delta+ cells results from the polyclonal expansion of pre-existing clones. Surprisingly, although the fetal thymocyte populations contain higher numbers of TcR gamma/delta+ cells than the adult and newborn ones, the highest proliferative response to IL 1 and IL 2 is obtained with the newborn thymocytes. Non mutually exclusive hypotheses are discussed to explain these results.