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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

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Updated: Jul 7, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Separation efficiencies in hydrophilic interaction chromatography.

Tohru Ikegami1, Kouki Tomomatsu, Hirotaka Takubo

  • 1Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ikegami@kit.ac.jp

Journal of Chromatography. A
|February 26, 2008
PubMed
Summary

Hydrophilic interaction chromatography (HILIC) is crucial for separating polar compounds. This review examines HILIC column efficiency, comparing particle-packed and monolithic columns for enhanced separation science.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Hydrophilic interaction chromatography (HILIC) is vital for separating highly polar substances.
  • These compounds include biologically active molecules like drugs, neurotransmitters, and carbohydrates.
  • HILIC utilizes polar stationary phases with aqueous organic mobile phases.

Purpose of the Study:

  • To review and discuss the column efficiency of HILIC materials.
  • To analyze the relationship between solute structure, stationary phase properties, and column performance.
  • To compare the efficiency of particle-packed versus monolithic HILIC columns.

Main Methods:

  • Literature review of HILIC materials and their performance.
  • Analysis of column efficiency based on solute and stationary phase characteristics.
  • Comparative study of particle-packed and monolithic column technologies.

Main Results:

  • Column efficiency in HILIC is influenced by solute properties and stationary phase chemistry.
  • Both particle-packed and monolithic columns offer distinct advantages in HILIC separations.
  • Recent literature data (2006-2007) provides insights into HILIC advancements.

Conclusions:

  • Understanding HILIC column efficiency is key to optimizing the separation of polar compounds.
  • The choice between particle-packed and monolithic columns depends on specific application needs.
  • Continued research in HILIC materials promises improved analytical capabilities.