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Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
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Controlled nerve growth factor release from multi-ply alginate/chitosan-based nerve conduits.

Lukas A Pfister1, Eva Alther, Michaël Papaloïzos

  • 1Institute of Pharmaceutical Sciences, Zurich, Switzerland.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
|February 26, 2008
PubMed
Summary

Researchers developed a novel nerve conduit (NC) system to control the release of nerve growth factor (NGF) for peripheral nerve regeneration. This multi-ply system offers sustained bioactive NGF delivery for at least 15 days.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Neuroscience

Background:

  • Peripheral nerve regeneration is crucial after injury.
  • Growth factor delivery kinetics significantly impact nerve repair.
  • Current methods for controlled nerve growth factor release are limited.

Purpose of the Study:

  • To design and characterize a multi-ply nerve conduit (NC) with adjustable release kinetics for nerve growth factor (NGF).
  • To optimize in vitro release of bioactive NGF for sustained delivery.
  • To evaluate the suitability of the developed NC for future nerve regeneration studies.

Main Methods:

  • Fabrication of a multi-ply nerve conduit using alginate/chitosan complex coated with poly(lactide-co-glycolide) (PLGA).
  • Optimization of release test media (citrate/acetate buffers with Tween 20) for NGF quantification via ELISA.
  • In vitro assessment of NGF release kinetics based on its radial location within the NC.

Main Results:

  • Optimized buffer conditions (citrate pH 5.0, acetate pH 5.5 with 0.05% Tween 20) ensured reliable ELISA quantification of active NGF.
  • Embedding NGF between two PLGA layers with sealed ends provided reproducible and controlled release.
  • Adjusting NGF's radial position allowed fine-tuning of release kinetics.
  • Sustained release of bioactive NGF (low ng/day) was achieved for over 15 days.

Conclusions:

  • The developed multi-ply NGF-loaded NC demonstrates tunable and sustained release of bioactive growth factors.
  • This system is a promising candidate for investigating the link between local growth factor availability and peripheral nerve regeneration.
  • Further in vivo studies are warranted to validate the efficacy of this nerve conduit in promoting nerve repair.