Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An ELISA assay for GAP-43.

W S Chang1, C H Friedman, M A Iqbal

  • 1Cephalon, Inc., West Chester, PA 19380.

Journal of Neuroscience Methods
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same journal

Pupil-DLC: an open-source deep learning pipeline for scalable, marker-less tracking of pupil dynamics across conscious and unconscious states.

Journal of neuroscience methods·2026
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
See all related articles

A new ELISA assay accurately measures growth-associated protein (GAP-43) in neuronal tissue. This method reveals high GAP-43 levels in developing rat brains, decreasing to adult levels by one month post-birth.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Growth-associated protein (GAP-43) plays a crucial role in neuronal development and plasticity.
  • Quantifying GAP-43 levels is essential for understanding neural development and regeneration.

Purpose of the Study:

  • To develop and validate a rapid Enzyme-Linked Immunosorbent Assay (ELISA) for quantifying growth-associated protein (GAP-43).
  • To assess the relative abundance of GAP-43 in developing rat brain tissue using the developed ELISA.

Main Methods:

  • Development of an ELISA assay utilizing an affinity-purified anti-GAP-43 antibody.
  • Validation of the assay through Western blot analysis of rat brain and liver homogenates.
  • Determination of GAP-43 quantity in as little as 0.6 micrograms of protein.

Related Experiment Videos

  • Assessment of assay reproducibility and inter-assay variation.

    • Main Results:

    • The ELISA assay specifically detected GAP-43 in rat brain homogenates, with no cross-reactivity in liver homogenates.
    • The assay demonstrated high reproducibility with low sample-to-sample ( < 5%) and inter-assay (2-7%) variation.
    • GAP-43 immunoreactivity in developing rat brain was 3-4 times higher at post-natal day 1 compared to adults.
    • Elevated GAP-43 levels persisted for several weeks, declining to adult levels by the end of the first month.

    Conclusions:

    • A reliable and sensitive ELISA assay for GAP-43 quantification in neuronal tissue has been established.
    • The assay provides a rapid method for assessing GAP-43 abundance, crucial for neuroscience research.
    • The study confirms the dynamic expression pattern of GAP-43 during early neuronal development in rats.