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Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Drug Dissolution: Requirements and Profile Comparison01:14

Drug Dissolution: Requirements and Profile Comparison

The acceptance criteria for dissolution profile data are anchored in Q values, representing the percentage of drug dissolved within a specified period. This assessment unfolds in three stages:First Stage: The test passes if all six drug dosage units are equal to or greater than Q plus 5%; otherwise, the sample proceeds to the second stage.Second Stage: The average of twelve units must be equal to or greater than Q, with no unit falling below Q - 15% to pass; if not, it progresses to the final...
Pharmaceutical Equivalents01:26

Pharmaceutical Equivalents

As defined by regulatory standards, pharmaceutical equivalents require generic drug products to have identical dosage forms and chemically identical active pharmaceutical ingredients (APIs). They must adhere to compendial or applicable standards for potency, content uniformity, disintegration times, and dissolution rates. In the case of modified-release dosage forms, variations in drug content are permissible as long as the delivered amount remains consistent with the innovator drug product.
Bioequivalence: Overview01:16

Bioequivalence: Overview

Pharmaceutical equivalents, by definition, are drug products with the same active ingredient in the same quantities, encapsulated in identical dosage forms, and intended for the same administration routes. These pharmaceutical equivalents are deemed bioequivalent if the bioavailability of the active entity in the drug preparations is similar. Moreover, pharmaceutical equivalents demonstrating bioequivalence are also regarded as therapeutically equivalent. This means that when used as directed,...

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Curtain Flow Column: Optimization of Efficiency and Sensitivity
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Published on: June 12, 2016

Comparison of two column characterisation systems based on pharmaceutical applications.

Erik Haghedooren1, Tamás Németh, Sanja Dragovic

  • 1Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Analyse, O&N 2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.

Journal of Chromatography. A
|February 26, 2008
PubMed
Summary

Choosing the right chromatographic column can be difficult. This study found that both the KUL and Euerby column characterization systems provide similar rankings for selecting reversed-phase liquid chromatography columns.

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Selecting appropriate chromatographic columns is crucial for reproducible analysis, especially when brand-specific stationary phases are not permitted in official monographs.
  • The vast number of commercially available reversed-phase liquid chromatography (RPLC) columns presents a significant challenge for chromatographers.
  • Existing column characterization systems aim to aid in rational column selection.

Purpose of the Study:

  • To compare the effectiveness of two column characterization systems: the KUL system and the Euerby system (Column Selector by ACD/Labs).
  • To evaluate the similarity in column rankings generated by these two distinct characterization approaches.

Main Methods:

  • The KUL system, utilizing 4 chromatographic parameters and 3 methods, and the Euerby system, based on 6 test parameters and 4 methods, were compared.
  • A set of 63 RPLC columns was analyzed.
  • Column rankings were generated using an F-value (KUL) or Column Difference Factor (Euerby) against a reference column for 7 pharmaceutical separations.

Main Results:

  • Both the KUL and Euerby methods produced similar column rankings.
  • While not identical, the two systems yielded highly comparable results, enabling confident selection of similar or dissimilar columns relative to a reference.
  • The KUL method requires fewer parameters and chromatographic methods, leading to a shorter analysis time for column characterization.

Conclusions:

  • The KUL and Euerby column characterization systems offer reliable and comparable outcomes for selecting RPLC columns.
  • The KUL system presents a more time-efficient approach due to its simpler methodology.
  • Both systems facilitate informed column selection, aiding chromatographers in overcoming the challenge of numerous available RPLC columns.