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Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...

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Related Experiment Video

Updated: Jul 7, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Improved multiplex-PCR and microarray for herpesvirus detection from CSF.

Anne J Jääskeläinen¹, Heli Piiparinen, Maija Lappalainen

¹Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland. anne.jaaskelainen@helsinki.fi

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology

|February 26, 2008

Summary

This summary is machine-generated.

This study enhanced a multiplex PCR and microarray method for detecting herpesviruses. The improved assay shows increased sensitivity for herpes simplex virus (HSV) and varicella-zoster virus (VZV) detection in clinical samples.

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Last Updated: Jul 7, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

Published on: November 4, 2025

Area of Science:

Molecular Biology
Virology
Clinical Diagnostics

Background:

A multiplex PCR and microarray method was previously developed for detecting eight herpesviruses in clinical specimens.
Herpesviruses, including herpes simplex virus (HSV) and varicella-zoster virus (VZV), are significant human pathogens.

Purpose of the Study:

To enhance a multiplex PCR and microarray assay for improved detection of herpes simplex virus (HSV) and varicella-zoster virus (VZV).
To update and validate the improved method using positive cerebrospinal fluid (CSF) samples.

Main Methods:

Design of a new primer pair for HSV PCR and novel detection oligonucleotides for HSV, VZV, CMV, EBV, HHV-6, and HHV-7.
Parallel testing of the revised multiplex PCR and microarray method against the previous version using serial dilutions of herpesvirus DNA and 20 positive CSF specimens.

Main Results:

The updated method demonstrated enhanced sensitivity for detecting herpes simplex viruses (HSV).
Newly designed detection oligonucleotides for VZV performed effectively even at low viral DNA concentrations.

Conclusions:

The revised HSV PCR and new HSV and VZV oligonucleotides function effectively and offer improved sensitivity.
The updated method increases the reliability of herpesvirus detection in clinical specimens.