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Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
Published on: May 16, 2025
Combination of poly(ethylenimine) and chitosan induces high gene transfection efficiency and low cytotoxicity
Qing-Qing Zhao1, Jin-Liang Chen, Min Han
1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Abstract:
In this study, the cytotoxicity and transfection efficiency of using chitosan/DNA complex combined with poly(ethylenimine) (PEI) were investigated. The combination of PEI with the chitosan/DNA complex markedly enhanced the gene expression of HeLa cells to 1000-fold of that induced by chitosan alone. PEI's cytotoxicity was considerably decreased upon combination with the chitosan/DNA complex. Furthermore, the PEI/chitosan/DNA could maintain the gene expression efficiency in the presence of serum.
