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Updated: Jul 7, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Screening and investigation of dye decolorization activities of basidiomycetes
Kouichi Nozaki1, Chee Hai Beh, Masahiro Mizuno
1Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, Nagano, Japan. knoza@shinshu-u.ac.jp
Abstract:
The decolorization of industrial dyes was investigated using extracellular enzymes produced by 21 basidiomycetes, mainly edible mushrooms. Among the 27 dyes used in this study, nine were decolorized by over 40%. Most fungi decolorized Acid Orange 20, but they showed different specificities in the case of the other dyes. Determination of activity staining by native polyacrylamide gel electrophoresis revealed that all the decolorization activities corresponded to 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation activities.
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