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Updated: Jul 7, 2026

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
Published on: February 11, 2021
Regional differences in taste bud distribution and alpha-gustducin expression patterns in the mouse fungiform papilla
Gen-Hua Zhang1, Hai-Yun Zhang, Shao-Ping Deng
1Sensory Science Laboratory, Department of Biological Science, Changshu Institute of Technology, 99 Nansanhuan Road, Changshu 215500, Jiangsu Province, People's Republic of China. zgh@cslg.edu.cn
Abstract:
The regional differences between distribution patterns and alpha-gustducin expression patterns of the fungiform (FF) taste buds were investigated in the adult mouse, using hematoxylin-eosin staining and immunofluorescence histochemistry on the most anterior region of the tongue (the first millimeter) through the intermediate region of the tongue (the last 1-4 mm). Paraffin sections were prepared from the tip to posterior regions (anterior and intermediate region containing the FF taste buds) of the adult mouse tongue. Results indicate that there were significant regional differences in size and density of taste buds, the cell counts of the single taste bud, and the alpha-gustducin-immunoreactive taste buds between the 2 regions. The taste bud had a characteristic onion-like appearance, and the alpha-gustducin-immunoreactive cell was spindle shaped with elongated processes extending from the base to the pore of the taste buds. These results provide a detailed insight to better understand regional descriptions of mouse taste bud density and size and alpha-gustducin expression with the mouse model.
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