Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

Biochemical and biophysical research communications·2026
Same author

Folding the unfoldable 2: using AlphaFold and ESMFold to explore spurious proteins.

Bioinformatics advances·2026
Same author

InterProScan 6: a modern large-scale protein function annotation pipeline.

Bioinformatics advances·2026
Same author

Viral non-coding RNA structure annotation and API-based data retrieval with Rfam and R2DT.

bioRxiv : the preprint server for biology·2026
Same author

Heart-nosed bat alphacoronaviruses use human CEACAM6 to enter cells.

Nature·2026
Same author

Integrative multi-omics defines melanoma drug response networks and ARID1A-dependent resistance mechanisms.

Molecular systems biology·2026

Related Experiment Video

Updated: Jul 7, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Large-scale screening for novel low-affinity extracellular protein interactions.

K Mark Bushell1, Christian Söllner, Benjamin Schuster-Boeckler

  • 1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.

Genome Research
|February 26, 2008
PubMed
Summary

Scientists developed AVEXIS, a new assay to map extracellular protein interactions. This method detects transient interactions, creating the first systematic network of low-affinity extracellular protein interactions.

More Related Videos

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Related Experiment Videos

Last Updated: Jul 7, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Extracellular protein-protein interactions mediate intercellular communication and tissue cohesion.
  • Current protein interaction datasets are incomplete, lacking membrane-tethered and secreted proteins due to technical limitations.
  • Existing methods struggle with hydrophobic transmembrane regions and transient extracellular interactions (half-lives < 1 second).

Purpose of the Study:

  • To develop a high-throughput assay for detecting transient extracellular protein interactions.
  • To overcome the limitations of existing methods in mapping extracellular interactomes.
  • To systematically screen for receptor-ligand pairs and build a comprehensive extracellular interaction network.

Main Methods:

  • Developed AVEXIS (avidity-based extracellular interaction screen), a high-throughput assay.
  • AVEXIS is designed to detect very transient interactions (half-lives ≤ 0.1 sec) with a low false-positive rate.
  • Systematically screened for receptor-ligand pairs within the zebrafish immunoglobulin superfamily.

Main Results:

  • Identified novel ligands for both known and orphan receptors in the zebrafish immunoglobulin superfamily.
  • Demonstrated that genes encoding identified receptor-ligand pairs are often phylogenetically clustered and co-expressed.
  • Generated the first systematic low-affinity extracellular protein interaction network, validated by independent biological data.

Conclusions:

  • AVEXIS successfully overcomes technical challenges in detecting transient extracellular interactions.
  • The developed method enables large-scale extracellular protein interaction mapping in diverse experimental settings.
  • This work provides a foundation for a more complete understanding of extracellular communication and function.