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Alteration of integrin-associated protein (CD47) on experimental porcine eperythrozoonosis
1College of Veterinary Medicine, China Agricultural University, Haidian District, Beijing 100094, China.
Abstract:
To evaluate the alteration of CD47 on RBCs of pigs infected with M. suis, we induced the experimental porcine eperythrozoonosis and collected the blood samples at the different time points. The result of analysis by flow cytometry after reaction with mouse-anti-human CD47 and caprine-anti-mouse IgG-FITC reagents indicated that the CD47 quantity on RBCs changed correlatively with the course of PE. The lowest value of M1 (percentage of the positive cells in fluorescence intensity) occurred on day 7 post inoculation at the peak of parasitemia and decreased 82% compared with the control sample. And then, the values of M1 on day 14 and 21 rised slowly but were still significantly different with the controls (p < 0.01). These suggested that the quantity of CD47 on RBCs altered progressively with the phases of the PE disease. The decrease of CD47 on RBCs maybe weaken the inhibitory CD47-SIRPalpha interaction and provide positive signals for phagocytosis of macrophages resulting in the removal of the RBCs from the circulation. In conclusion, CD47, a marker on RBCs, maybe play an important role on the mechanism of hemolysis caused by infection of M. suis.
Insights
In pigs infected with Mycoplasma suis, CD47 levels on red blood cells (RBCs) significantly decreased during infection, potentially leading to increased RBC removal. This finding highlights CD47
Area of Science:
- Veterinary Medicine
- Immunology
- Parasitology
Background:
- Mycoplasma suis (M. suis) causes porcine eperythrozoonosis (PE), a significant disease in swine.
- Red blood cell (RBC) destruction (hemolysis) is a hallmark of PE, but the underlying mechanisms require further elucidation.
- CD47 is a cell surface protein involved in immune regulation, particularly in preventing phagocytosis.
Purpose of the Study:
- To investigate the changes in CD47 expression on RBCs of pigs experimentally infected with M. suis.
- To explore the potential role of CD47 alterations in the pathogenesis of porcine eperythrozoonosis and subsequent hemolysis.
Main Methods:
- Experimental infection of pigs with M. suis.
- Blood sample collection at various time points post-inoculation.
- Flow cytometry analysis using anti-CD47 antibodies to quantify CD47 expression on RBCs.
Main Results:
- CD47 quantity on RBCs significantly decreased during M. suis infection, reaching a minimum 82% reduction by day 7 post-inoculation.
- CD47 levels remained significantly lower than control values on days 14 and 21, indicating a progressive alteration.
- The observed decrease in CD47 correlated with the progression of porcine eperythrozoonosis and parasitemia.
Conclusions:
- CD47 expression on RBCs is dynamically altered during M. suis infection.
- The reduction in CD47 may impair the CD47-SIRPalpha inhibitory signaling pathway.
- This impairment could promote macrophage-mediated phagocytosis of RBCs, contributing to the hemolysis observed in porcine eperythrozoonosis.
