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Updated: Jul 7, 2026
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Published on: December 12, 2011
[Effects of two different cryoprotectants on the motility of post-thaw human sperm]
Jing-Mei Hu1, Cheng-Yan Xu, Yuan Li
1Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China.
Objective:
To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm.
Methods:
The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups.
Results:
The forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants.
Conclusion:
Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.
