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Updated: Aug 3, 2026

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
Published on: November 11, 2016
Protein kinase inhibitor, H-7, directly affects N-methyl-D-aspartate receptor channels
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.
Abstract:
Compounds commonly used to modify protein phosphorylation have been found to interact directly with K channels, Ca channels, and acetylcholine receptor channels. We report that a general protein kinase inhibitor, H-7, directly affects N-methyl-D-aspartate (NMDA) receptors independent of protein kinase activity. Single-channel records in the presence of H-7 have fewer bursts of activity, and the currents are interrupted in a voltage-dependent manner by brief closures. Hippocampal whole-cell currents induced by NMDA are not decreased by H-7, in part, because the single-channel currents have longer burst lengths that compensate for the brief closures.
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