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Inhibition of Cdk Activity

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Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells.

Pei-chang Wang¹, Zong-yu Zhang, Jian Zhang

¹Department of Medical Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100053, China. peichangwang@yahoo.com

Chinese Medical Journal

|February 27, 2008

Summary

This summary is machine-generated.

Astragali Radix compounds HDTIC-1 and HDTIC-2 significantly inhibit p16 expression, delaying cellular senescence in human fibroblasts. These compounds also exhibit anti-oxidative properties, contributing to their anti-aging effects.

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Area of Science:

Gerontology
Molecular Biology
Traditional Chinese Medicine

Background:

Astragali Radix is a traditional Chinese anti-aging herb.
HDTIC-1 and HDTIC-2 are isomers extracted from Astragali Radix.
Previous studies showed HDTIC compounds delay fibroblast replicative senescence.

Purpose of the Study:

Investigate the effects of HDTIC-1 and HDTIC-2 on senescence-associated gene expression.
Explore the mechanism by which HDTIC compounds delay replicative senescence.

Main Methods:

RT-PCR and Western blot were used to observe p16 and p21 expression.
Phenotype alteration after antioxidant treatment assessed anti-oxidative activities.

Main Results:

HDTIC compounds significantly reduced p16 mRNA and protein expression in senescent cells.
p21 expression increased with cell aging, unaffected by HDTIC compounds.
HDTIC compounds restored non-senescent phenotype in H2O2-damaged cells.

Conclusions:

HDTIC compounds inhibit p16 expression, contributing to delayed senescence via the p16(INK4a)/Rb/MAPK pathway.
Anti-oxidative activities of HDTIC compounds may be linked to p16 inhibition.