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Cytoprotective effect of baicalein against peroxynitrite-induced toxicity in LLC-PK(1) cells
Xiang-Lan Piao1, Eun Jung Cho, Moon Hee Jang
1Chinese Minority Traditional Medicine Center, College of Life and Environment Sciences, Central University for Nationalities, Beijing, China.
Abstract:
The various reactions of peroxynitrite (ONOO(-)) with enzymes, macromolecules and lipids, have been shown to influence cellular functions and may be involved in the pathogenesis of various diseases. Therefore, targeting ONOO(-)-induced cytotoxicity is now accepted as a viable strategy to alleviate disease signs in numerous diseases. There is a growing interest in the antioxidant characteristics and use of dietary flavonoids in the management of ONOO(-)-related diseases. Baicalein, a major bioactive flavonoid constituent of Scutellaria baicalensis Georgi, possesses a multitude of pharmacological activities that have been associated with its antioxidant effects. In this study, we have investigated the ONOO(-) scavenging ability of baicalein and its protective effect on ONOO(-)-induced cell death in cultured LLC-PK(1) cells. Baicalein was able to scavenge ONOO(-) in a concentration-dependent manner, which was comparable to penicillamine. In addition, baicalein at the concentration range of 10-50 microM increased cell viability and decreased LDH leakage in 3-morpholinosydnonimine (SIN-1)-treated cells, reflecting its prevention of ONOO(-)-induced cytotoxicity. These results suggest that the protective effects of baicalein can be explained with respect to its ability to scavenge ONOO(-) and may be useful as a possible therapeutic strategy for the treatment of involved diseases.
Insights
Baicalein, a flavonoid, effectively scavenges peroxynitrite (ONOO(-)) and protects cells from ONOO(-)-induced damage. This suggests baicalein
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Peroxynitrite (ONOO(-)) contributes to cellular dysfunction and disease pathogenesis.
- Targeting ONOO(-)-induced cytotoxicity is a therapeutic strategy for various diseases.
- Dietary flavonoids are explored for their antioxidant properties in managing ONOO(-)-related conditions.
Purpose of the Study:
- To investigate the peroxynitrite (ONOO(-)) scavenging ability of baicalein.
- To evaluate baicalein's protective effects against ONOO(-)-induced cell death in LLC-PK(1) cells.
Main Methods:
- Assessed baicalein's ONOO(-) scavenging capacity in vitro.
- Utilized LLC-PK(1) cells treated with 3-morpholinosydnonimine (SIN-1) to induce ONOO(-) production.
- Measured cell viability and lactate dehydrogenase (LDH) leakage to assess cytotoxicity.
Main Results:
- Baicalein demonstrated concentration-dependent ONOO(-) scavenging, comparable to penicillamine.
- Baicalein (10-50 microM) significantly increased cell viability in SIN-1 treated cells.
- Baicalein reduced LDH leakage, indicating prevention of ONOO(-)-induced cytotoxicity.
Conclusions:
- Baicalein possesses significant peroxynitrite (ONOO(-)) scavenging properties.
- Baicalein protects cells from ONOO(-)-induced cytotoxicity.
- Baicalein may serve as a therapeutic agent for diseases associated with ONOO(-) damage.