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Examination of Pyroptosis by Flow Cytometry
Published on: May 31, 2024
cGnRH II involvement in pyriform cell apoptosis.
Stefania Tammaro1, Palma Simoniello, Silvana Filosa
1Department of Biological Sciences, Evolutionary and Comparative Biology Unit, University of Naples Federico II, Via Mezzocannone 8, 80134 Naples, Italy.
Cell and Tissue Research
|February 27, 2008
Summary
Gonadotrophin-releasing hormone (GnRH) triggers nurse cell apoptosis in lizards. This hormone activates DNase I, an alternative cell death pathway, but not Fas/Fas-L or caspases.
Area of Science:
- Reproductive biology
- Cellular biology
- Endocrinology
Background:
- Nurse cells (pyriforms) are crucial for oocyte growth in previtellogenesis.
- The role of gonadotrophin-releasing hormone (GnRH) in regulating nurse cell apoptosis is not well understood.
Purpose of the Study:
- To investigate the involvement of GnRH in triggering nurse cell (pyriform) apoptosis during lizard oogenesis.
- To elucidate the specific pathway through which GnRH may exert its apoptotic effects.
Main Methods:
- Immunocytochemical analysis to detect GnRH receptors in pyriform cells.
- In vitro treatment of pyriform cells with cGnRH II.
- Assays for DNAse I activation, DNA laddering, Fas/Fas-L synthesis, and caspase activation.
Main Results:
- Pyriform cells express GnRH receptors, upregulated by cGnRH II during late previtellogenesis.
- cGnRH II treatment induced DNAse I activation and DNA laddering, indicating apoptosis.
- No significant induction of Fas/Fas-L synthesis or caspase activation was observed.
Conclusions:
- cGnRH II acts as a pro-apoptotic factor for pyriform cells in Podarcis sicula.
- The apoptotic effect is mediated through an alternative pathway, likely involving calcium and DNase I, independent of the Fas/Fas-L and caspase pathways.
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