Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analytical Ultracentrifugation in Aotearoa: A Brief History of the Technique and its Place Among Other Tools Used to Investigate Biomolecular Interactions.

Journal of the Royal Society of New Zealand·2026
Same author

Introducing CLipPA lipid chemodiversity to enzymatically truncated polymyxin B: a soft drug strategy to combat Gram-negative pathogens.

RSC medicinal chemistry·2026
Same author

Natural Killer T follicular helper cell function permits germinal centre entry in nasal associated lymphoid tissue.

Mucosal immunology·2025
Same author

Rapid and Stereoselective Access to 6″-Amino-6″-deoxy-α-GalCer Scaffolds.

The Journal of organic chemistry·2025
Same author

Mechanistic insight into the induction of liver tissue-resident memory CD8<sup>+</sup> T cells by glycolipid-peptide vaccination.

Cell reports·2025
Same author

Natural and Semisynthetic Immunomodulatory Luakuliide Labdane Diterpenoids.

Journal of natural products·2024

Related Experiment Video

Updated: Jul 7, 2026

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
07:04

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Published on: September 16, 2018

Phosphatidylinositol mannosides are efficient mucosal adjuvants.

Natalie A Parlane1, Michel Denis, Wayne B Severn

  • 1AgResearch, Hopkirk Research Institute, AgResearch Grasslands, Palmerston North, New Zealand.

Immunological Investigations
|February 27, 2008
PubMed
Summary

Mycobacterial phosphatidylinositol mannosides (PIMs) enhance cell-mediated immunity, acting as novel adjuvants for vaccines. These PIMs stimulate Th1 responses and mucosal immunity, crucial for fighting intracellular pathogens.

More Related Videos

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
10:06

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

Related Experiment Videos

Last Updated: Jul 7, 2026

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
07:04

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Published on: September 16, 2018

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
10:06

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

Area of Science:

  • Immunology
  • Vaccinology
  • Microbiology

Background:

  • Defined sub-unit vaccines necessitate immunological adjuvants to enhance immune responses.
  • Adjuvants promoting cell-mediated immunity, specifically Th1 responses, are crucial for vaccines targeting intracellular bacteria like Mycobacterium tuberculosis.

Purpose of the Study:

  • To assess the adjuvant potential of mycobacterial phosphatidylinositol mannosides (PIMs) in modulating cell-mediated immune responses.
  • To evaluate PIMs' ability to enhance immune responses against a model antigen, ovalbumin (OVA), using transgenic mice.

Main Methods:

  • Utilized transgenic mice with a high proportion of T cells specific for an OVA peptide.
  • Administered purified native or synthetic PIMs with OVA via subcutaneous, oral, or intranasal routes.
  • Assessed cellular immune responses by measuring cytokine release (IFN-gamma, IL-4, IL-12) and T cell activation.

Main Results:

  • PIMs significantly enhanced interferon-gamma (IFN-gamma) release, indicating a Th1-biased response.
  • Oral and intranasal administration of PIMs with OVA generated efficient cell-mediated immunity.
  • PIMs stimulated interleukin-12 (IL-12) release from dendritic cells (DCs).

Conclusions:

  • Purified and synthetic PIMs function as effective adjuvants, particularly for mucosal immunization.
  • PIMs represent a promising new class of adjuvants for developing vaccines against intracellular pathogens.
  • PIMs are potent stimulators of Th1 and IL-12 responses, crucial for cell-mediated immunity.